(298) Role of Donor-Derived Cell-Free DNA in Chronic Lung Allograft Dysfunction, a Longitudinal Study

PurposeSurvival after lung transplantation (LT) is hampered by the development of chronic allograft dysfunction (CLAD). There no treatment available to reverse CLAD once diagnosed, but early intervention could modify progressive function decline. Thus, biomarkers are need for detection CLAD. High levels donor derived cell-free DNA (ddcfDNA) have been described precede acute cellar rejection and antibody mediated diagnosis. The aim this study assess if there any association between ddcfDNA (CLAD).MethodsThis a longitudinal in which were determined 100 LT recipients at 3rd, 6th, 9th, 12th, 24th 36th month LT. Firstly, real-time PCR was performed detect an informative INDEL polymorphism each donor/recipient pair. Secondly, quantifying digital plasma recipient. Clinical data collected during three years follow-up determine infections, cellular rejection, CLAD.ResultsGlobally, we noted upward trend 1, 2 3 when referred months. Levels compared patients different time points, cut-off value 1.86% 12 months identify those with higher probability developing (Figure 1).ConclusionDetermine be useful non-invasive biomarker prediction. This partially funded ISCIII ("PI17/01485"), co-funded ERDF SEPAR (552/2017). Survival Globally, 1). Determine